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Ligand blot of P252 binding to Cry1A toxins. P252 was resolved by SDS-PAGE and transferred to a PVDF membrane. Samples from the SDS-PAGE gel (PAG-Plate) and the PVDF membrane (PVDF) were stained with CBB. Immobilized P252 was incubated with Cry1Aa, Cry1Ab, and Cry1Ac, and toxin binding was visualized using anti-Cry1Aa and anti-Cry1Ac.
