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Western blots of P252 using anti-APN-120K and anti-BtR175. P252 was resolved by SDS-PAGE and transferred to a PVDF membrane, and samples from the SDS-PAGE gel (PAG-Plate) and the PDVF membrane (PVDF) were stained with CBB. Western blotting was performed using anti-APN-120K or anti-BtR175, as indicated (top row). As controls, partially purified APN-120K (middle row) and BtR175 (bottom row) were also analyzed using the appropriate antibodies.
