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Lectin binding analysis of P252. (A) P252 was resolved by SDS-PAGE and transferred to a PVDF membrane, and samples from the SDS-PAGE gel (PAG-Plate) and the PDVF membrane (PVDF) were stained with CBB. Lectins (ConA, WGA, PHA-E4, LCA, PNA, and SBA) conjugated to peroxidase were incubated with the immobilized P252. (B) Schematic of typical oligosaccharide structures recognized by various lectins used in this experiment.
