FIG. 4.

TLC analysis of versicolorin A production in A. parasiticus. (A) The veA and ΔveA spread plate cultures were grown in the light or in the dark on solid YGT medium for 7 days. The numbers 1 to 4 represent replicates. (B) In a shift experiment, the veA and ΔveA cultures were incubated in liquid YGT for 24 h and then equal amounts of mycelium were shifted into solid medium (6 g/9-cm-diameter plate) and allowed to grow for an additional 96 h. The experiment was repeated twice with similar results (indicated as lanes 1 and 2). Versicolorin A standards (Std) are shown on both sides of the TLC plate.