TABLE 1.
Primers and probe sequences used in RTQ-PCR
| Target | Forward primer | Reverse primer | Probed |
|---|---|---|---|
| bssAa | 5′ACGACGGYGGCATTTCTC3′ | 5′GCATGATSGGYACCGACA3′ | FAM-5′CTTCTGGTTCTTCTGCACCTTGGACACC3′-TAMRA |
| Bacteriab | 5′CGGTGAATACGTTCYCGG3′ | 5′GGWTACCTTGTTACGACTT3′ | FAM-5′CTTGTACACACCGCCCGTC3′-BHQ-1 |
| Archeaec | 5′CGGTGAATACGTCCCTGC3′ | 5′AAGGAGGTGATCCTGCCGCA3′ | FAM-5′CTTGTACACACCGCCCGTC3′-BHQ-1 |
| 5′CGGTGAATATGCCCCTGC3′ | |||
| Phage (λ)a | 5′ACGCCACGCGGGATG3′ | 5′AGAGACACGAAACGCCGTTC3′ | TET-5′ACCTGTGGCATTTGTGCTGCCG3′-TAMRA |
a
The forward and reverse primer as well as the probe were designed by Beller et al. (7).
b
The forward primer BACT1369F, reverse primer PROK1492R, and probe TM1389F were developed by Suzuki et al. (53).
c
The forward primer ARCHMIX1369F (ARCH1-1369F and ARCH2-1369F), reverse primer PROK1541R, and probe TM1389F were developed by Suzuki et al. (53).
d
The reporter dye used was FAM (6-carboxyfluorescein) or TET (tetrachloro-6-carboxyfluorescein), and the quencher dye was either TAMRA (6-carboxy- tetramethyl rhodamine) or BlackHole Quencher-1.