FIG. 6.

RT-PCR expression analysis of PcDIP5 and PcGAP1 in P. chrysogenum grown under different conditions. Expression of PcDIP5 and PcGAP1 was determined after 24 h of growth on minimal medium containing glucose as the carbon source and different nitrogen sources (A) or on various media as indicated (B). Penicillin production medium (pen prod) contains urea and glutamate as nitrogen and carbon sources, respectively. The other three media are minimal media with lactose (lact) or glucose (gluc) as the sole carbon source and glutamate or urea as the sole nitrogen source. Isolated mRNA was used as a template for semiquantitative RT-PCR. The expression analysis provides relative information for one gene only; analyses for different genes should not be compared.