Figure 5. Overexpression of pri-OsamiR395h impacts tobacco sulfate transportation and distribution.

(a) Statistical analysis of total sulfur in leaf and root tissues. Samples were harvested from four weeks old wild type and three transgenic tobacco lines. Data are presented as means of three biological replicates contains mixed samples from five biological replications, error bars represent SD (n = 3). (b) Statistical analysis of sulfate-S concentrations in leaf and root tissues. Samples were harvested from four weeks old wild type plants and three transgenic tobacco lines. Data are presented as means of fifteen biological replicates, error bars represent SD (n = 15). The statistically significant difference between groups was determined by one-way ANOVA (F(df_between, df_within) = F ration, p = p-value, where df = degrees of freedom). Means not sharing the same letter are statistically significantly different (P < 0.05). (c) Statistical analysis of sulfate concentration in tobacco leaves of different ages. Leaves of 12 weeks old wild type and three transgenic tobacco lines were harvested in the positions as indicated in the figure. Data shown are an average of three biological replicates, error bars represent SD (n = 3). DW: dry weight. FW: fresh weight. WT: wild type.