Figure 8. NtamiR395 and NtaSULTR2 exhibit opposite expression patterns in tobacco roots.

Real-time PCR analysis of expressions of NtaSULTR2 and mature NtamiR395 under different sulfate concentrations. Total RNA samples were prepared from (a) leaf tissue and (b) root tissue of four weeks old tobacco grown in MS medium with 0, 20, 1500 or 2000 μM (NH⁴⁺)₂SO₄. NtaL25 was used as a reference gene. Data are presented as means of three technical replicates and two biological replicates, error bars represent SD (n = 6). The statistically significant difference between groups was determined by one-way ANOVA (F(df_between, df_within) = F ration, p = p-value, where df = degrees of freedom). Means not sharing the same letter are statistically significantly different (P < 0.05).