FIG. 4.

Arsenic selectivity. (A) The intracellular metal or As contents of resting cells with or without overexpression of ELP153AR (control) were determined after 1 h of incubation with either As(III) (10 μM), As(V) (10 μM), cadmium chloride (25 μM), or zinc chloride (65 μM). (B) Effect of sodium ion on As(III) binding. Resting cells were resuspended in Tris buffer (pH 7.4) containing 10 μM As(III), and the indicated concentration of NaCl was added. As(III) binding was determined after 1 h of incubation. Data shown are the mean values (± standard deviations) obtained from three independent experiments.