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. 2016 Jun 28;6:28706. doi: 10.1038/srep28706

Figure 2. CAMSAP3/Marshalin re-organizes cytoskeletal networks.

Figure 2

(A–C) Immunofluorescent images showing MT bundles induced by CAMSAP3/Marshalin-Ld. A merged image (C) shows both CAMSAP3/Marshalin-Ld (A) and MT staining (B). (D–F) CAMSAP3/Marshalin-Ld-induced MT bundles disappeared when cells were treated with 43 nM colchicine. A merged image (F) shows CAMSAP3/Marshalin-Ld (D) and tubulin (E) staining. (G–J) Co-transfection of GFP with CAMSAP3/Marshalin-Ld does not interfere with thick bundle formation. A merged image (J) shows CAMSAP3/Marshalin-Ld (G), Tubulin (H), and GFP (I). (K–N) MT bundles disappeared when cells were co-transfected with CAMSAP3/Marshalin-Ld and CDH23-C1-GFP. A merged image (N) shows CAMSAP3/Marshalin-Ld (K), Tubulin (L), and CDH23-C1-GFP (M). CAMSAP3/Marshalin-Ld was stained using anti-Marshalin (A,D,G,K); MTs by anti-α-tubulin (B,E,H,L).