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. 2016 Jun 28;6:28833. doi: 10.1038/srep28833

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Copyright © 2016, Macmillan Publishers Limited

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SGC-996 and GBC-SD cells were transfected with vector control (NC) or shSTMN1-1. 72 h later, the protein of each cell line was extracted. The expression of STMN1 in the two cell lines was detected by WB (a) and qRT-PCR (b), respectively. Then, shSTMN1-1 was selected randomly for further research. The growth curve was tested by MTS assay (c). Migration (d) and invasion (e) assays of SGC-996 and GBC-SD cells were performed 24 h after incubated. Results were shown as means ± SEM from three independent experiments. NS: Non-significant; NC: Negative control. *p < 0.05; **p < 0.01. Significance was determined using Student’s t-test.