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. 2016 Jun 28;6:28693. doi: 10.1038/srep28693

Table 1. Oligonucleotide primer sequences used for quantitative-RT-PCR.

Gene Forward primer Reverse primer Reference Notes
Pck2 ATCCGAAAGCTCCCCAAGTAC CAATCACCGTCTTGCTTTCTACTC    
Pck1 CTGAGCCACATGGAGGAAGAG GATACCGTCTTGCTTTCAATTC    
Phgdh CTGGCCGGCGTTGTAAAC GCTTCAGCCAGACCAATCCA    
Psat1 CAAAGTGCAGGCTGGAAATAACT CCCCGCCGTTGTTCTTAA    
Psph GGGCATAAGGGAGCTGGTAAG GACGGGATGTTGAGCTTTGAA    
Fgf21 TCCAGACATCCCGGTTCCT AAACGTTGTAGCCATCCTCAAGA   Q.RT.PCR
Fgf21 ACCTCTACACGGATGATGCC CCATCCTCAAGAAGCAGCTC   RT.PCR
Pkm1 ACCGCAAGCTGTTTGAAGAA TCCATGAGGTCTGTGGAGTG    
Pkm2 GAGGCCTCCTTCAAGTGCT CCAGACTTGGTGAGGACGAT    
Myh7 AAGGGCTTGAACGAGGAGTAGA TTATTCTGCTTCCTCCAAAGGG Wimmers et al.59 Annealing temperature 57 °C
Myh2 GCTGAGCGAGCTGAAATCC ACTGAGACACCAGAGCTTCT
Myh1 AGAAGATCAACTGAGTGAACT AGAGCTGAGAAACTAACGTG
Myh4 ATGAAGAGGAACCACATTA TTATTGCCTCAGTAGCTTG
Eno3 CATGAGGATTGAGGAGGCTCTT GGCCTTTGGGTTACGGAACT    
Idh2 TTCATCAAGGAGAAGCTCATCCT TGGTCTGGTCCCGGTTTG