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. 2016 Jun 28;6:28769. doi: 10.1038/srep28769

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Copyright © 2016, Macmillan Publishers Limited

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(A) Dose-dependent inhibitory effect of G-rich oligonucleotides. Primers Olig2F and Olig2R were used to amplify their 2 kb target. The inhibitory effect of the additional oligonucleotides Stat3R and GfapR were diminished when their final concentration is below 0.1 μM. (B) Lowering the final concentration of the inhibitory primer enables it to amplify its target. Stat3F and various amount of Stat3R were used to amplify their target. Only when the Stat3R were used at concentration of 0.133 μM or below can the target be amplified efficiently. (C) Inhibitory effect of G-rich sequences can be reduced by their complementary sequences. Additional oligonucleotides GGGGGG and CCCCCC were added to see the yield of the PCR products using Olig2F and Olig2R as primers.