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GPC of chitosan hydrolysates. Twenty milligrams of chitosan solution/ml was hydrolyzed by chitosanase (2 U/g of chitosan) at 40°C for 36 h. During the reaction, aliquots were sampled and their Mavs were estimated by GPC (Ultrahydrogel 250; Waters). Molecular weight indicates pullulan standard P-82 (48,000, P-50; 12,200, P-10; 5,800, P-5) and predicted molecular weight (1,600 and 960) from a pullulan molecular weight standard curve.
