TABLE 1.

Summary of the purification steps of chitosanase from Bacillus sp. strain KCTC 0377BP

Purification step	Total protein (mg)	Total activity (U)	Sp act (U/mg)	Purification factor	Yield (%)
Culture supernatant	162	11,700	72	1	100
PEG concentration	82	10,500	130	2	90
CM-Toyopearl 650 M	7.5	8,500	1,100	15	73
Superose 12HR FPLC	3.1	5,400	1,700	24	46