TABLE 2.
Substrate specificity of the chitosanase from Bacillus sp. strain KCTC 0377BP
| Substrate | Relative activity (%)a |
|---|---|
| Chitosan oligosaccharides | |
| Chitobiose | ND |
| Chitotriose | ND |
| Chitotetraose | 15.0 |
| Chitopentaose | 16.8 |
| Chitohexaose | 20.0 |
| Chitoheptaose | 23.8 |
| Soluble chitosan | 100.0 |
| Colloidal chitin | 2.8 |
| CMC | 2.5 |
| Peptidoglycan | ND |
| Soluble starch | ND |
a
A total of 0.5% (wt/vol) of substrates were used for chitosanase assay. Relative activity was expressed as rates relative to the activity of enzyme on soluble chitosan. ND, not detectable.