TABLE 4.
Comparison of properties of purified chitosanases produced by microorganismse
| Microorganism | Mol mass (kDa)a | Action modef | Optimum pH | Sp. act (U/mg) | Substrate specificity (% DDA)b | Inducibilityc | End productsd | Source or reference |
|---|---|---|---|---|---|---|---|---|
| Bacillus sp. strain KCTC 0377BP | 45 | Endo | 4.0-6.0 | 1,700 | Chitosan (40-100), CMC (2.5) | C | G3-G7 | This study |
| Aspergillus oryzae IAM2660 | 40 | Endo | 5.5 | 17.2 | Chitosan (70-100) | C | Oligomer | 51 |
| 135 | Exo | 5.5 | 38.8 | Chitosan (90-100) | I | G1 | ||
| Nocardia orientalis IFO 12806 | 97 | Exo | 5.5 | 35.8 | Chitosan (100) | I | G1 | 28 |
| Acinetobacter sp. strain CHB101 | 37 | Endo | 5-9 | 334 | Chitosan (70-90) | C | G2, G3 | 39 |
| 30 | Endo | 5-9 | 800 | Glycol chitosan | C | |||
| Enterobacter sp. strain G-1 | 50 | Exo | 7.0 | 5.84 | Chitosan (80) | I | G2 | 49 |
| Myxobacter strain AL-1 | 31 | Endo | 5.0, 6.8 | ND | CMC, chitosan | ND | ND | 32 |
| Matsuebacter chitosanotabidus 3001 | 34 | Endo | 4.0 | 250 | Chitosan (90) | I | G1-G3 | 31 |
| B. cereus S1 | 45 | Endo | 6.0 | 196 | Chitosan (ND), CMC (21.6) | C | G2-G4 | 20 |
| B. megaterium P1 chitosanase A | 43 | Endo | 4.5-6.5 | 154.8 | Chitosan (81), CMC (0.4) | I | Oligomer | 33 |
| Bacillus sp. strain KFB-C108 | 48 | Endo | 6.5 | 110 | Chitosan (ND) | I | G3-G5 | 50 |
a
Determined by SDS-PAGE analysis with purified enzyme.
b
Substrate specificity for chitosan and CMC. Data in parentheses indicate appropriate DDA of chitosan for the activity (more than 50% relative activity) and relative activity of CMC for chitosan.
c
C and I are abbreviations for constitutive and inducible, respectively.
d
End product of the enzymes using chitosan as the substrate.
e
ND, not determined.
f
Exo, exocleavage type; endo, endocleavage type.