Figure 1.

Effects of bisphenol A (BPA) with, and without, 17beta-estradiol (E2) and triiodo-thyronine (T3), on ERβ mRNA expression. BPA treatment decreased ERβ mRNA expression compared to the non-treated control. In glia-reduced cultures (Glia−), a more than two orders of magnitude higher (×180) ERβ mRNA expression was found in BPA treated cultures (column B vs. F). In glia containing samples (Glia+), there was about three magnitudes higher ERβ mRNA expression after E2 + T3 + BPA (column D) treatment than after BPA treatment alone (column B). Letters in and above the columns indicate significant differences (p ≤ 0.05) from columns below.