Figure 5.

Effects of co-treatments with bisphenol A (BPA), zearalenone (Zea), arsenic (As), and methylbenzylidene camphor (MBC) with, and without 17beta-estradiol (E2) and triiodo-thyronine (T3) on ERβ mRNA expression. There was a robust and significant increase in ERβ mRNA expression in AllEDs treatment group of glia containing (Glia+) cultures, when compared to the ntC. However, high variance in differences were also found. In contrast, no increase was found in glia-reduced (Glia−) samples. ERβ mRNA expression values in E2 + T3 + All EDs groups were nearly identical in Glia+ vs. Glia− cultures. Compared to their respective non-treated controls, transcript levels in E2 + T3 + AllEDs cultures doubled in Glia+, while decreased to the one third in Glia−. Letters above the columns indicate significant differences (p ≤ 0.05) from columns below.