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. 2016 Jun 28;6:28990. doi: 10.1038/srep28990

Figure 7.

Figure 7

Immunofluorescent staining with (a) the claudin-1 antibody in wild-type C57BL/6 mice fed a high fat diet (HFD) or normal chow diet (Con) for 22 weeks and treated with recombinant IL-22 20 ng/g or 100 ng/g i.p twice weekly) for the last 4 weeks; staining intensity as determined by ImageJ analysis. (b) Endotoxin levels (EU/mL) in the serum collected from mice at the end of the experiment. (c) Levels of goblet cell transcription factor Klf4 and goblet cell mucin Muc2 were determined by qRT-PCR. Normalized to expression of β-Actin and expressed as a fold change of the mean of control. (d) Immunohistochemical staining for Muc2 precursor and quantification as a percentage of total crypt area using ImageJ software. Data presented are mean ± SEM. N = 8–12 animals per group. One-Way ANOVA with Bonferroni post-test Con versus HFD and HFD versus IL-22 treatment. *p < 0.05 **p < 0.01 ***p < 0.001. Scale bars = 50 μM.