Table 2.

Phenotypic microarray (PM) wells in which the growth of bioluminescent C. rodentium derivative ICC180 significantly differs from its non-bioluminescent parent strain ICC169.

PM class	Substrate	Adjusted P value	Improved growth by ICC169	Improved growth by ICC180	Comment
Nitrogen	D-glucosamine	0.0159		√
	Cytidine	0.0280		√
	Ala-His	0.0316		√
Phosphate	Inositol hexaphosphate	0.0280		√
Nitrogen peptides	Lys-Asp	0.0306	√
Chemicals	Kanamycin	0.0076		√	Conferred by KanR gene
	Paromomycin	0.0048		√	Aminoglycoside—the kanamycin cassette will be mediating resistance
	Geneticin	0.0048		√	Aminoglycoside—the kanamycin cassette will be mediating resistance
	Dequalinium chloride	0.0116		√	Quaternary ammonium salt
	Spiramycin	0.0088		√	Macrolide—acts at ribosomal 50S, c.f. aminoglycosides at 30S
	Rolitetracycline	0.0316		√	Tetracycline; prevents tRNA binding at 30S A-site
	Doxycycline	0.0210		√	Tetracycline; prevents tRNA binding at 30S A-site
	Coumarin	0.0333		√	Fragrant organic compound found in many plants
	Iodonitro tetrazolium violet (INT)	0.0087		√	Electron acceptor, reduced by succinate dehydrogenase (and by superoxide radicals)
	EDTA	0.0048	√		Metal chelator
	EGTA	0.0210	√		Metal chelator
	Rifampicin	0.0048	√		RNA polymerase inhibitor
	Colistin	0.0048	√		Cyclic polypeptide; disrupts outer membrane
	Oxycarboxin	0.0121	√		Fungicide
	Phenethicillin	0.0048	√		Beta-lactam
	Cytosine-1-b-D-arabinofuranoside	0.0123	√		Nucleoside analogue (anti-cancer/-viral)
	Sodium Nitrate	0.0306	√
	Cefoxitin	0.0316		√	Beta-lactam
	Disulphiram	0.0349		√	Inhibits acetaldehyde dehydrogenase