FIG. 2.

Analysis of cotranscription of the tst and sbp1 genes of A. ferrooxidans by RT-PCR. (A) Schematic map of the genetic context around the tst and sbp1 genes of A. ferrooxidans ATCC 23270. Arrows indicate locations of primers used for the RT reaction and for PCR (a through f). The genes present in the region analyzed were p14.3 and tst, encoding TST-like proteins; tox1, encoding a terminal oxidase subunit; sbp1, encoding an SBP; hyp, encoding a hypothetical protein; and cdt, encoding a C₄-dicarboxylate transporter. (B) Agarose gel electrophoresis of RT-PCR products obtained for the tst, tox1, and sbp1 intergenic regions. The primers used in each reaction are indicated (a through f). The RT reaction was carried out on 2 μg of total RNA obtained from sulfur-grown cells of A. ferrooxidans. RT reactions with (+ lanes) and without (− lanes) the Moloney murine leukemia virus reverse transcriptase enzyme were carried out in order to exclude amplification due to genomic DNA contamination. Sizes of DNA markers are shown on the left. Expected sizes (in base pairs) for the corresponding RT-PCR products are given below the gel.