FIG. 3.

Characterization of some proteins from total cells or different fractions from A. ferrooxidans. Omp44 was identified in outer membrane-enriched fractions from cells grown on ferrous iron (A) or sulfur (B). P30 was identified in soluble fractions obtained from cells grown on ferrous iron (C) or sulfur (D). Cytochrome c₅₅₂ was identified in total-cell proteins from cells grown on ferrous iron (E) or sulfur (F). Sulfate binding protein (spot 2) and PstS (spot 1) were identified in total proteins from cells grown on ferrous iron (G) or thiosulfate (H). All proteins were separated by 2-D NEPHGE, and spots were stained with Coomassie blue (A, B, C, D, G, and H) or with heme-staining reagent (E and F).