FIG. 5.

Western blot analysis of rusticyanin in A. ferrooxidans grown under different conditions. (A) Equivalent amounts of total-cell proteins from A. ferrooxidans grown on ferrous iron (lane a), elemental sulfur (lane b), copper sulfide (lane c), zinc sulfide (lane d), or pyrite (lane e) were used for immunodetection with anti-Rus antibodies. (B) Quantification of the bands shown in panel A by using the Scion Image processing program. Values shown are relative to those obtained for ferrous iron-grown cells.