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Synthesis of rusticyanin, putative protein 7, and TST during growth of A. ferrooxidans on pyrite or chalcopyrite. Cells were grown on pyrite (A) or chalcopyrite (B), total-cell proteins were separated by 2-D PAGE, and the proteins were stained with Coomassie blue. Only the section of the gel containing the proteins analyzed is shown. Arrow indicates the spot corresponding to pancreatic RNase, used to treat the samples for 2-D PAGE.
