Fig. 1.

The Effect of Different Hesperetin Concentrations in LPDM on the Activity of pLDLr Luc + in Human Hepatoma HepG2 Cells after 24 h. HepG2 Cells were transfected with 1 μg/well human pLDLr driving firefly luciferase. To assay the pLDLr promoter response to the different concentrations of hesperetin, 5 mg/ml LPDM + 2 μl/ml DMSO was used as vehicle. Cells were exposed to 25 μM, 50 μM, 100 μM, 150 μM and 200 μM hesperetin in fresh 5 mg/ml LPDM. After incubation for 24 h, cells were harvested for luciferase and protein assays. pGL3 Luciferase reporter-Basic and -Control vectors were used to indicate the transfection efficiency. The difference between groups was evaluated by one-way ANOVA with Dunnett’s multiple comparisons test. The data represent an individual experiment, which was repeated twice. Values were expressed as mean ± SEM; n = 3 per treatment