Table 2.
The primer sequences of target and internal reference genes used in qPCR
| Gene | Forward primer | Reverse primer | GenBank accession | PCR products |
|---|---|---|---|---|
| LAP | AGGCTCCATCACCTGCTCCTT | CCTGCAGCATTTTACTTGGGCT | AY911374.1 | 183 bp |
| BNBD5 | CTGGATCACCTGCTCCTCGT | GGTGCCAATCTGTCTCATGTTG | NM_001130761.1 | 150 bp |
| IL-1β | GGCCAAAGTCCCTGACCTCT | CTGCCACCATCACCACATTC | NM_174093.1 | 167 bp |
| IL-6 | GGAGGAAAAGGACGGATGCT | GGTCAGTGTTTGTGGCTGGA | NM_173923.2 | 227 bp |
| IL-8 | CCTCTTGTTCAATATGACTTCCA | GGCCCACTCAATAACTCTC | NM_173925.2 | 170 bp |
| TNF-α | CTTCTGCCTGCTGCACTTCG | GAGTTGATGTCGGCTACAACG | AF_348421.1 | 156 bp |
| NF-kB | ATACGTCGGCCGTGTCTAT | GGAACTGTGATCCGTGTAG | NM_001045868.1 | 129 bp |
| GAPDH | GGGTCATCATCTCTGCACCT | GGTCATAAGTCCCTCCACGA | XM_001252479 | 177 bp |