TABLE 1.
Sampling method | Material | Culture analysis (CFU/sample)
|
QPCR analysis (templates/sample)
|
||
---|---|---|---|---|---|
Pre | Post | Pre | Post | ||
Heavy wipe | Vinyl tile | 6.43 × 105 | 5.41 × 105 | 3.37 × 105 | 4.47 × 105 |
Wood laminate | 7.97 × 105 | 8.43 × 105 | 1.42 × 105 | 4.52 × 105 | |
Metal cabinet | 5.62 × 105 | 6.77 × 105 | 2.50 × 105 | 4.39 × 105 | |
Swipe | Vinyl tile | 7.45 × 105 | 7.79 × 105 | 6.15 × 105 | 9.48 × 105 |
Wood laminate | 1.56 × 106 | 7.85 × 105 | 6.16 × 104 | 7.54 × 105 | |
Metal cabinet | 7.81 × 105 | ND | 5.95 × 105 | 6.66 × 105 | |
SSP kit | Vinyl tile | 4.55 × 105 | 5.16 × 105 | 1.04 × 106 | 1.23 × 106 |
Wood laminate | 9.06 × 105 | 4.02 × 105 | 1.26 × 106 | 1.33 × 106 | |
Metal cabinet | 5.83 × 105 | 5.20 × 105 | 1.15 × 106 | 1.22 × 106 |
Data were obtained in a control experiment with no chlorine dioxide gas decontamination to determine if physical factors during treatment affected postdecontamination results. The control experiment was conducted in an identical manner as the other B. atrophaeus decontamination trials, with the exception that chlorine dioxide gas was not released into the room. Surface samples (929 cm2, [1 ft2]; n = 1 for each material) were collected with Heavy Wipes, swipes, and SSP kits prior to treatment (Pre) and posttreatment (Post). Culture and QPCR results were obtained for the detection of B. atrophaeus from surfaces in the experimental room. ND, not determined.