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Hu-IFN-α2b yield obtained from each gene variant at 5 h postinduction. In the insert, the first four lanes in the Western blots correspond to the standard hu-IFN-α, 50, 100, 200, and 400 ng, respectively. Lane 5 corresponds to the 5-h sample of each gene variant: IFN (5 μl; OD = 1.96), MR1 (2 μl; OD = 2.58), MR2 (10 μl; OD = 1.50), MR12 (4 μl; OD = 1.37), MR3 (5 μl; OD = 1.50), MR13 (1 μl; OD = 1.80) and MR123 (1 μl; OD = 2.72) l., liter.
