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. 2016 Jun 27;9:361. doi: 10.1186/s13071-016-1647-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Standardization of RT-qPCR assays for gene expression analysis of immunological markers. Golden hamsters were infected with 10⁵ Leishmania (V.) braziliensis promastigotes, for 110 days. The panel shows a representative amplification plot with the fluorescent signal magnitude (a) and melting curve indicating the reaction specificity, observed through a single peak in each curve (b). Calibration curves of γ-Actin, IFN-γ, GAPDH, TNF, TGF-β, Arginase, iNOS, IL-10, IL-4 and IL-6 target genes from golden hamster indicates the linearity of the reaction (c). RT-qPCR assays were performed using SYBR Green fluorophore, as described in Methods section