Table 1.

Primers and standard curve parameters for immunological marker amplification in golden hamsters infected by Leishmania (V.) braziliensis

Golden hamster gene target	Primer sequences	Reference	Amplicon length	Slope	Intercept	Coefficient of linearity (r2)	Amplification efficiency (%)
GAPDH	Fw 5′-GGT TGC CAA ACC TTA TCA GAA ATG-3′	Ribeiro-Romão et al. [11]	194 bp	-3.30	20.03	0.98	100.7
	Rv 5′-TTC ACC TGT TCC ACA GCC TTG-3′
ɣ Actin	Fw 5′-ACA GAG AGA AGA TGA CGC AGA TAA TG-3′	Espitia et al. [18]	70 bp	-3,28	20.06	0.99	101.7
	Rv 5′-GCC TGA ATG GCC ACG TAC A-3′
IFN-ɣ	Fw 5′-TGT TGC TCT GCC TCA CTC AGG-3′	Espitia et al. [18]	130 bp	-3.48	26.03	0.99	93.4
	Rv 5′-AAG ACG AGG TCC CCT CCA TTC-3′
TGF-β	Fw 5′-GGC TAC CAC GCC AAC TTC TG-3′	Espitia et al. [18]	81 bp	-3.52	27.11	0.99	92.3
	Rv 5′-GAG GGC AAG GAC CTT ACT GTA CTG-3′
TNF	Fw 5′-TGA GCC ATC GTG CCA ATG-3′	Espitia et al. [18]	79 bp	-3.33	24.81	0.99	99.5
	Rv 5′-AGC CCG TCT GCT GGT ATC AC-3′
IL-10	Fw 5′-GGT TGC CAA ACC TTA TCA GAA ATG-3′	Espitia et al. [18]	194 bp	-3.21	29.66	0.99	104.7
	Rv 5′-TTC ACC TGT TCC ACA GCC TTG-3′
IL-4	Fw 5′-CCA CGG AGA AAG ACC TCA TCT G-3′	Zivcec et al. [25]	72 bp	-2.98	31.06	0.98	116.4
	Rv 5′-GGG TCA CCT CAT GTT GGA AAT AAA-3′
IL-6	Fw 5′-GGA CAA TGA CTA TGT GTT GTT AGA A-3′	This study	99 bp	-3.23	28.85	0.97	103.8
	Rv 5′- AGG CAA ATT TCC CAA TTG TAT CCA G-3′
iNOS	Fw 5′-TGA GCC ACT GAG TTC TCC TAA GG-3′	Osorio et al. [26]	93 bp	-3.54	29.71	0.95	92.7
	Rv 5′-TCC TAT TTC AAC TCC AAG ATG TTC TG-3′
Arginase	Fw 5′-ACC TAT GTG TCA TTT GGG TGG A-3′	Osorio et al. [26]	163 bp	-3.38	20.57	0.99	97.5
	Rv 5′-GCA GAT ATG CAG GGA GTC ACC-3′