. 2016 Jun 28;17:94. doi: 10.1186/s12863-016-0399-9

Table 6.

Epistatic effects and interacting effects of epistatic QTL × environment of identified QTL for traits related to flag leaf morphology of main shoots in the RIL population

Trait	QTL_i	Flanking marker	Position(cM)	QTL_j	Flanking marker	Position (cM)	Environ.	AA	h ²(AA)%	AAE	h ²(AAE)%
FLL	Qfll.acs–1A.1	Xmag1022–Xwmc24	0	Qfll.acs–1A.2	Xwmc93–Xcfd25	0	E3	0.55***	8.25	–0.25**	3.50
	Qfll.acs–1B.1	Xwmc694–Xwmc156	0	Qfll.acs–2D.1	Xmag1280–Xgwm157	2	E1	–0.37***	2.43
	Qfll.acs–1B.3	Xwmc830–Xwmc44	2	Qfll.acs–3B.2	Xgwm566–Xwmc231	2	E3	–0.30***	2.51
	Qfll.acs–1D	Xbarc169–Xwmc216	2	Qfll.acs–7A	Xwmc596–Xgwm260	2	E4	0.34***	2.06	–0.39***	2.94
	Qfll.acs–2A.1	Xwmc474–Xwmc296	4	Qfll.acs–4D.4	Xwmc399–Xwmc622	2	E3	0.35***	3.43
	Qfll.acs–2A.2	Xgwm122–Xmag2150	0	Qfll.acs–4A.3	Xwmc757–Xgwm613	0	E4	–0.42***	3.27	0.33***	4.05
	Qfll.acs–2B.2	Xbarc167–Xmag3698	0	Qfll.acs–6B.2	Xbarc24–Xpsp3131	4	E3	0.41***	4.67	–0.32***	4.08
	Qfll.acs–2D.1	Xmag1280–Xgwm157	2	Qfll.acs–3A.4	Xwmc695–Xgwm162	0	E1	–0.40***	2.93	–0.36***	2.42
	Qfll.acs–3A.3	Xwmc11–Xgwm391	6	Qfll.acs–5A.4	Xgwm186–Xcfa2185	0	E4	–0.49***	4.35	–0.50***	5.11
	Qfll.acs–3B.1	Xwmc236–Xmag3356	0	Qfll.acs–4A.4	Xgwm397–Xgwm613	0	E2	–0.36***	2.78
	Qfll.acs–4A.2	Xgwm160–Xwmc497	4	Qfll.acs–7B	Xwmc526–Xwmc232	2	E1	–0.47***	3.95	–0.41***	3.86
	Qfll.acs–4B	Xbarc1133–Xbarc90	4	Qfll.acs–1A.3	Xgwm135–Xwmc304	0	E4	–0.45***	3.73	0.31***	3.42
	Qfll.acs–4D.2	Xwmc489–Xgdm61	0	Qfll.acs–1A.4	Xwmc20–Xbarc240	2	E1	0.36***	3.61	–0.29***	4.73
							E3	0.42***	4.71
	Qfll.acs–4D.3	Xwmc473–Xwmc489	0	Qfll.acs–2A.3	Xgwm558–Xbarc208	8	E4	0.35***	2.21
	Qfll.acs–6A.1	Xwmc553–Xwmc179	0	Qfll.acs–6A.2	Xbarc113–Xwmc621	0	E4	0.40***	2.91
FLW	Qflw.acs–1A.1	Xgwm33–Xwmc818	0	Qflw.acs–1A.2	Xwmc611–Xwmc20	12	E3	0.03***	2.01	0.02***	3.41
	Qflw.acs–1B	Xbarc131–Xgwm413	2	Qflw.acs–3B.1	Xgwm108–Xpsp3035	0	E3	–0.03***	2.12	–0.03***	2.35
	Qflw.acs–1D	Xwmc429–Xwmc339	4	Qflw.acs–7A.2	Xksum153–Xwmc607	6	E2	–0.02***	2.36
	Qflw.acs–2A.3	Xgwm512–Xgwm30	0	Qflw.acs–5B.3	Xmag959–Xwmc740	0	E4	0.02***	2.05	–0.02***	2.28
	Qflw.acs–3A.3	Xwmc505–Xwmc343	0	Qflw.acs–2D	Xwmc112–Xgwm484	0	E4	0.02***	2.27
	Qflw.acs–3A.4	Xwmc50–Xksum222	6	Qflw.acs–3B.2	Xwmc366–Xgdm64	0	E2	0.03***	3.05
	Qflw.acs–4A	Xgwm613–Xmag3733	10	Qflw.acs–7D	Xgdm67–Xmag892	0	E2	0.02***	2.36	–0.02***	2.50
	Qflw.acs–5A.1	Xwmc492–Xgwm96	0	Qflw.acs–5B.3	Xmag959–Xwmc740	2	E1	0.02***	2.05	0.01***	2.23
FLWR	Qflwr.acs–1A.1	Xcfa2513–Xksum104	0	Qflwr.acs–4D	Xwmc399–Xwmc622	0	E1	0.20***	2.18	0.16**	2.84
	Qflwr.acs–1A.2	Xgdm36–Xbarc83	0	Qflwr.acs–7D	Xgwm121–Xgdm67	0	E2	0.29***	4.18	–0.31***	4.62
	Qflwr.acs–1B.2	Xbarc61–Xwmc134	10	Qflwr.acs–2D.2	Xmag1280–Xgwm157	0	E1	–0.29***	4.56
	Qflwr.acs–2B	Xwmc272–Xgwm630	0	Qflwr.acs–4B.2	Xbarc90–Xgwm540	2	E3	–0.23***	2.86	–0.19***	2.82
	Qflwr.acs–2D.1	Xwmc243–Xcfd239	0	Qflwr.acs–5D	Xcfd183–Xwmc212	2	E2	–0.38***	7.28	–0.30***	4.99
	Qflwr.acs–3A.3	Xwmc264–Xgwm494	0	Qflwr.acs–4A.2	Xgwm613–Xmag3733	6	E1	0.39***	7.67
							E2	0.29***	4.36
	Qflwr.acs–3B.1	Xbarc173–Xgwm284	0	Qflwr.acs–1B.3	Xgwm413–Xwmc419	12	E3	0.39***	5.93	–0.40***	5.05
	Qflwr.acs–3B.2	Xgdm64–Xwmc51	0	Qflwr.acs–3A.4	Xgwm67–Xwmc264	2	E4	0.21***	2.45	–0.23***	2.64
	Qflwr.acs–4A.1	Xwmc757–Xgwm610	2	Qflwr.acs–4A.3	Xgwm397–Xgwm613	0	E4	–0.26***	3.73	–0.22***	3.20
	Qflwr.acs–4B.1	Xksum244–Xmag2055	2	Qflwr.acs–5B	Xcfd10–Xbarc59	2	E4	–0.23***	2.73	0.18**	3.31
	Qflwr.acs–6A.2	Xgwm169–Xwmc580	2	Qflwr.acs–7B	Xbarc315–Xwmc311	2	E4	0.21***	2.29
	Qflwr.acs–6B.1	Xwmc539–Xmag590	0	Qflwr.acs–6B.2	Xbarc79–Xgwm626	0	E2	0.31***	4.95	–0.35***	4.27
FLA	Qfla.acs–1A.1	Xwmc104–Xcfa2219	14	Qfla.acs–1D	Xcfd72–Xwmc429	0	E2	0.62***	3.08	–0.35***	1.93
	Qfla.acs–2A.1	Xwmc474–Xwmc296	2	Qfla.acs–2B.2	Xgwm55–Xbarc128	0	E1	–1.39***	2.61	0.66***	1.18
	Qfla.acs–2A.2	Xgwm249–Xcfa2263	0	Qfla.acs–4D.3	Xgdm61–Xwmc457	18	E3	–0.74***	2.33	–0.68***	2.51
	Qfla.acs–2B.1	Xbarc1072–Xwmc272	0	Qfla.acs–6A.2	Xbarc113–Xwmc621	0	E3	1.16***	5.75	–0.94***	6.02
	Qfla.acs–3D	Xwmc43–Xwmc675	16	Qfla.acs–5D	Xcfd183–Xwmc212	4	E3	0.77***	3.56	–0.73***	3.34
	Qfla.acs–4A	Xmag3733–Xwmc707	0	Qfla.acs–7D	Xgdm67–Xmag892	0	E2	0.57***	2.58	–0.62***	2.85
	Qflaacs–5A	Xwmc205–Xgwm154	4	Qfla.acs–1A.2	Xgwm135–Xwmc304	0	E4	–0.87***	3.27
BAFL	Qbafl.acs–1D	Xbarc169–Xwmc216	4	Qbafl.acs–7A.1	Xwmc596–Xgwm260	0	E3	1.79***	2.03	–1.85***	2.18
							E4	2.72***	4.72	–1.84***	4.31
	Qbafl.acs–2B.2	Xbarc1155–Xcfd73	4	Qbafl.acs–3B.2	Xbarc68–Xgwm285	0	E1	1.94***	2.61	–1.75***	2.48
	Qbafl.acs–3A.1	Xwmc695–Xgwm162	0	Qbafl.acs–2B.3	Xgwm132–Xcfa2278	0	E2	–2.64***	2.58
	Qbafl.acs–3B.1	Xbarc1077–Xwmc366	2	Qbafl.acs–7A.2	Xgwm282–Xmag828	4	E2	–2.35***	2.05	–1.67***	2.06
	Qbafl.acs–4A.1	Xwmc420–Xgwm601	2	Qbafl.acs–4A.2	Xgwm610–Xgwm397	2	E4	–1.85***	2.19	–1.72***	2.34
	Qbafl.acs–4B.1	Xksum238–Xcfd39	0	Qbafl.acs–4D.2	Xksum180–Xwmc48	10	E1	–1.93***	2.57
	Qbafl.acs–4B.2	Xbarc292–Xbarc1133	0	Qbafl.acs–1A	Xbarc197–Xcfa2513	0	E4	–2.38***	3.62	–1.83***	4.29
	Qbafl.acs–5A	Xwmc630–Xmag4263	2	Qbafl.acs–3B.3	Xwmc291–Xgwm108	6	E3	2.37***	5.72	–1.94***	4.32
	Qbafl.acs–6A	Xgwm427–Xwmc642	0	Qbafl.acs–3A.2	Xwmc532–Xgwm674	14	E1	1.83***	2.32

FLL flag leaf length, FLW flag leaf width, FLWR flag leaf length to width ratio, FLA flag leaf area, BAFL basal angle of flag leaf. E1 to E4 represent the location at Yongdeng (103°18’ E, 36°42’ N), Gansu, China, in 2011and 2012, at Anning (103°51’ E, 36°04’ N), Gansu, China, in 2012, and at Yuzhong (104°07’ E, 35°51’ N), Gansu, China, in 2013, respectively. QTL_i and QTL_j are a pair of QTL detected by two–dimensional searching. AA: the direction of the epistatic effect; a positive value means that the parent–type effect is greater than the recombinant–type effect and a negative value means that the parent–type effect is less than the recombinant–type effect. h ²(AA) (%) : the phenotypic variance explained by epistatic QTL. AAE, a positive value indicates AAE effect having an increasing effect on the trait value in WW conditions and a negative value means AAE effect having a decreasing effect on the trait value in DS conditions. h ²(AAE) (%):the phenotypic variance explained by AAE effect. **P ≤0.005, ***P ≤0.001