Fig. 1.

The level of miR-1269b was increased in HBV-HCC cells by pre-miR-1269b, which was up-regulated by HBx-activated NF-κB. a The relative expression levels of miR-1269b, pre-miR-1269b and pre-miR-1269a in HepG2 and HepG2.2.15 cells were detected using real-time PCR. b The relative expression level of miR-1269b in HepG2 and SMMC-7721 cells transfected with pFLAG or pFLAG-HBx. c HBx promoted the nuclear translocation of NF-κB nucleus, as confirmed by western blot analysis. d The relative levels of miR-1269b and pri-miR-1269b were up-regulated in cells transfected with NF-κB compared to their levels in cells that were transfected with the control vector. All error bars indicate the mean standard deviation based on three independent experiments. *p < 0.05, **p < 0.01