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. 2016 Jun 27;9:38. doi: 10.1186/s13048-016-0248-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Validation of CRAd-mediated expression of IL-24 and ING4 genes. a) The concentrations of IL-24 protein following infection of the indicated OvCa cell lines and normal IOSE523 cells with CRAd-IL24 at the MOIs of 1 and 10 vp/cell were determined in cell culture supernatants 3 days postinfection using commercial ELISA kit with IL-24 concentration standards. Each bar represents the cumulative mean ± SD (*p ≤ 0.05). b) The relative levels of ING4 gene expression were determined following infection of SKOV3ip.1 cells with CRAd-ING4 at the MOIs of 100, 33, and 11 vp/cell. The ING4 protein band of 29 kDa was detected in cell lysates 3 days postinfection with 100, 33, and 11 vp/cell, but not in mock-infected cells (0 vp/cell) using Western blot with rabbit polyclonal Ab raised against ING4 internal region