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. 2016 Jun 27;9:38. doi: 10.1186/s13048-016-0248-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Evaluation of oncolytic effects of CRAd-IL24 or CRAd-ING4 alone and combined together. Monolayers of SKOV3ip.1, SKOV3luc, OVCAR3, and OV-4 cells were infected with each CRAd alone (left panel) or two CRAd vectors together (right panel) at the indicated MOIs. Oncolytic effects of CRAd vectors were assessed by determining cell monolayer integrity 6 days postinfection. The integrity of cell monolayers was determined by staining adherent cells with crystal violet. The stained monolayers were scanned using a plate reader set at 565 nm to calculate the percentage cell density in monolayers infected with each viral dose that were determined with respect to the uninfected control. Each data point represents the cumulative mean ± SD (error bars are smaller than the symbols, p ≤ 0.05)