Figure 2.

Timing of replication initiation. Examples of mutants/plasmids with altered initiation (I^P; green) and sequestration (S^P; blue) periods. The horizontal line represents one doubling time, whereas the vertical (hyphenated) line illustrates the time of initiation of the first origin in wild-type cells. Note that the start of S^P always coincides with the first origin initiated, i.e., start of I^P. In the graphical representation of initiation synchrony, the number of origins per cell are on the X-axis, whereas the cell number is on the Y-axis of each histogram. When more than one mutation/plasmid is listed for a specific example (e.g., in C,E–G,I), the histograms are representative of the initiation phenotype of each individual mutation/plasmid.