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. 2004 Aug;70(8):4971–4979. doi: 10.1128/AEM.70.8.4971-4979.2004

FIG. 2.

FIG. 2.

Flow diagram of the culture-independent approach to assess the abundance of T. denitrificans-like bacteria. Solid lines depict those characteristics which have to be measured for our quantification approach. Dotted lines depict characteristics which have to be calculated in order to estimate the numbers of T. denitrificans-like cells, 16S rRNA molecules, and ribosomes. Detailed calculations are given in Materials and Methods.