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. 2004 Aug;70(8):4971–4979. doi: 10.1128/AEM.70.8.4971-4979.2004

FIG. 4.

FIG. 4.

Detection limits of environmental T. denitrificans-like 16S rRNA in sample G3 (50-m depth) from the Baltic Sea by real-time PCR. (A) Sensitivity of the Com and OST primer sets in quantifying T. denitrificans-like 16S rRNA. After RT-PCR with primer 1492R and preamplification with primers 27F and 1492R, 16S rcDNA was used as the template in quantities of 7.8 × 1010 to 7.8 × 107 molecules for the Com primer set and 1.7 × 107 to 1.7 × 103 molecules for the OST primer set (values shown in italics), representing CT values ranging from 3.0 to 32.2. Norm. Fluoro., normal fluorescence. (B) Relation between the CT and the apparent amount of 16S rcDNA molecules obtained with SybrGreen real-time PCR. Each point represents an amount of 16S rcDNA molecules corresponding to the CT value with the Com and OST primer sets. The correlation coefficient of the regression line was 0.999. The PCR efficiency was 0.82.