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. 2004 Aug;70(8):4971–4979. doi: 10.1128/AEM.70.8.4971-4979.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 5. — Mean values of T. denitrificans-like 16S rRNA fractions (percent relative abundance) achieved from quantifications without (lane 1) and with (lane 2) a preamplification step. (A) Enrichment Bx21. The Student t test (P < 0.05) showed no evidence for a significant variation between results gained by using both quantification protocols (P value, 0.96; n = 15; degrees of freedom, 13). (B) Enrichment Bx24. The Student t test (P < 0.05) showed no evidence for a significant variation between results gained by both quantifications protocols (P value, 0.83; n = 8; degrees of freedom, 6).