TABLE 1.
Comparison of total 16S rRNA contents from seawater samples and enrichmentsa
| Sample | Depth (m) or source of enrichment (sample) | Total 16S rRNA content (ng/ml) | Total 16S rRNA (no. of molecules/ml)b |
T. denitrificans-like-organism-specific 16S rRNA fraction
|
Total cells (106/ml)c | T. denitrificans- like-organism- specific 16S rRNA gene fraction (% relative abundance) | No. of T. denitrificans-like cells/ml | No. of ribosomes/ T. denitrificans- like cell | |
|---|---|---|---|---|---|---|---|---|---|
| % Relative abundance | No. of molecules/ml | ||||||||
| G1 | 5 | 1.41 (±0.04) | 1.62 × 109 (±4.48 × 107) | 0.003 (±0.003) | 4.86 × 104 (±1.34 × 103) | 2.15 | 0.012 (±0.002) | 2.58 × 102 (±4.30 × 101) | 200 |
| G2 | 30 | 0.06 (±0.02) | 6.59 × 107 (±2.09 × 107) | 0.059 (±0.018) | 3.89 × 104 (±1.23 × 104) | 1.31 | — | — | — |
| G3 | 50 | 0.31 (±0.01) | 3.54 × 108 (±1.46 × 107) | 0.002 (±0.001) | 7.10 × 103 (±2.92 × 102) | 0.53 | — | — | — |
| G4 | 80 | 0.21 (±0.00) | 2.44 × 108 (±9.51 × 105) | 0.0036 (±0.0024) | 8.78 × 103 (±3.42 × 101) | 0.43 | 0.021 (±0.007) | 9.00 × 101 (±3.01 × 101) | 100 |
| G6 | 120 | 0.29 (±0.01) | 3.32 × 108 (±6.43 × 106) | 0.083 (±0.038) | 2.76 × 105 (±5.34 × 104) | 0.27 | 1.552 (±0.158) | 4.19 × 103 (±4.27 × 102) | 70 |
| G7 | 138 | 0.46 (±0.01) | 5.24 × 108 (±8.75 × 106) | 0.916 (±0.251) | 4.80 × 106 (±8.02 × 104) | 0.85 | 8.346 (±3.110) | 7.08 × 104 (±2.64 × 104) | 70 |
| G9 | 175 | 0.48 (±0.02) | 5.53 × 108 (±1.97 × 107) | 0.466 (±0.075) | 2.58 × 106 (±9.18 × 104) | 0.90 | 15.055 (±3.519) | 1.35 × 105 (±3.17 × 104) | 20 |
| G10 | 225 | 0.04 (±0.00) | 4.65 × 107 (±3.78 × 106) | — | — | 1.10 | — | — | — |
| Bx21 | G7 | 3.27 (±0.22) | 3.76 × 109 (±2.63 × 108) | 8.445 (±0.826) | 3.18 × 108 (±3.11 × 107) | 1.66 | 17.850 (±2.709) | 2.96 × 105 (±4.50 × 104) | 1,100d |
| Bx23 | G9 | 3.23 (±0.13) | 3.71 × 109 (±1.45 × 108) | 19.099 (±1.070) | 7.09 × 108 (±3.97 × 107) | 1.31 | 38.894 (±9.100) | 5.10 × 105 (±1.19 × 105) | 1,400e |
| Bx24 | G9 | 7.74 (±0.10) | 8.90 × 109 (±1.19 × 107) | 49.723 (±9.951) | 4.43 × 109 (±8.86 × 108) | 4.43 | 49.654 (±8.001) | 2.20 × 106 (±3.54 × 105) | 2,000f |
Total 16S RNA contents from seawater samples and enrichments with T. denitrificans-like-organism-specific relative and cellular 16S rRNA and 16S rRNA gene abundances and estimated number of ribosomes per T. denitrificans-like cell. Standard deviations are given in parentheses. —, below detection limit.
Based on 1.15 × 109 molecules of 16S rRNA/ng.
Data from Weinbauer et al. (44).
Value indicates a 16-fold increase of ribosome content per cell in comparison to that of the original environmental sample.
Value indicates a 70-fold increase of ribosome content per cell in comparison to that of the original environmental sample.
Value indicates a 100-fold increase of ribosome content per cell in comparison to that of the original environmental sample.