Figure 5.

Effect of upregulated miR‐24 on tumor radiosensitivity and SP1 miRNA expression. Prior to injection into BALB/C mice, CNE1 cells were transfected with miR‐24 mimic. Tumors were irradiated with a single dose of 12 Gy/1f/1d. (A). Tumor growth in irradiated mice. (B). Photographs of tumors from miR‐24 and control groups. (C) Expression of SP1 was determined by using quantity RT‐PCR in tumors. The results were represented as mean ±SD. *P < 0.05 compared with negative control. (D). Immunohistochemical staining and quantification of expression of Sp1 was determined. The cytoplasm was counter‐stained with hematoxylin. (E). Western blot analysis of SP1 in nude mice xenograft tumor tissue.