Table 3.

Primers used for Sanger sequencing

Gene	Primers
Adenomatous polyposis coli (APC) EXON 15 PART 1	Fw 5′‐TGAAGAGAAACGTCATGTGGA‐3′
	Rev 5′‐CTTTGCAAGTGGCAGCCTTT‐3′
APC EXON 15 PART 2	Fw 5′‐AAGTGGTCAGCCTCAAAAGG‐3′
	Rev 5′‐GTGACACTGCTGGAACTTCG‐3′
APC EXON 15 PART 3	Fw 5′‐GATCCTGTGAGCGAAGTTCC‐3′
	Rev 5′‐AACATGAGTGGGGTCTCCTG‐3′
APC EXON 15 PART 4	Fw 5′‐ACACCCAAAAGTCCACCTGA‐3′
	Rev 5′‐ACTTCTCGCTTGGTTTGAGC‐3′
APC EXON 15 PART 5	Fw 5′‐AGCTCAAACCAAGCGAGAAG‐3′
	Rev 5′‐TTTCCTGAACTGGAGGCATT‐3′
APC EXON 15 PART 6	Fw 5′‐GCCTCCAGTTCAGGAAAATG‐3′
	Rev 5′‐ACAGGCAGCTGACTTGGTTT‐3′
APC EXON 15 PART 7	Fw 5′‐AGCCCAGACTGCTTCAAAAT‐3′
	Rev 5′‐TGCCCCTCCTCTAACTCCTT‐3′
AKT1 EXON 2	Fw 5′‐CGAAGGTCTGACGGGTAGAG‐3′
	Rev 5′‐CGCCACAGAGAAGTTGTTGA‐3′
RET EXON 11	Fw 5′‐AGGGATAGGGCCTGGGCTTC‐3′
	Rev 5′‐GACCTGGTTCTCCATGGAGTC‐3′