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. 2016 Apr 5;5(6):1259–1267. doi: 10.1002/cam4.686

Figure 1.

Figure 1

Activation of γ‐Aminobutyric acid receptor (GABABR) inhibited the proliferation of colorectal cancer cell line HT29. (A) Baclofen inhibits HT29 proliferation detected by MTS proliferation assay. Ctrl means the cells treated with physiological saline. Data shown are means ± SD (n = 3). (B) BrdU incorporation assay showed the inhibition of proliferation caused by baclofen. Data shown are means ± SD (n = 4). *P < 0.05 versus the corresponding control. (C) Flow cytometry assay showed the cell cycle arrest at G1 phase by baclofen. Data shown are means ± SD (n = 5). (D) Expression level of mRNA of cell cycle‐related genes. Data shown are means ± SD (n = 4). *P < 0.05, ***P < 0.001 versus the corresponding control. (E) Detection of the proliferation of cells with downregualtion of GABABR. Data shown are means ± SD (n = 5). (F) BrdU incorporation assay of cells with downregualtion of assay GABABR. Data shown are means ± SD (n = 5). **P < 0.01 versus the corresponding control. (G) Cell cycle analysis by flow cytometry assay. Data shown are means ± SD (n = 5). (H) Expression level of mRNA of cell cycle‐related genes. Data shown are means ± SD (n = 4). **P < 0.01, ***P < 0.001 versus the corresponding control.