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. 2016 Apr 5;5(6):1259–1267. doi: 10.1002/cam4.686

Figure 2.

Figure 2

γ‐Aminobutyric acid receptor (GABABR) signaling repressed colorectal cell proliferation by inhibiting activity of GSK‐3β. (A) Representative picture showed the increasing level of phosphorylation level of GSK‐3β caused by activation of GABABR detected by western blot. (B) Downregulation of GABABR activates the GSK‐3β. (C) MTS proliferation assay showed the function of activation of GABABR can be blocked by activation of GSK‐3β by wort. Data shown are means ± SD (n = 4). (D) BrdU incorporation assay of the rescue experiment. Data shown are means ± SD (n = 4). ***P < 0.001 versus the corresponding control. (E) Flow cytometry assay. Data shown are means ± SD (n = 6). (F) The expression level of cell cycle‐related genes detected by qRT‐PCR. Data shown are means ± SD (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001 versus the corresponding control.