Table 1.

Amino acid sequences and molecular weights of M2pep analogs.

Name	Sequence	Mw (Calculated)	Mw (Observed)	Note
Linear M2pepBiotin analogs
M2pepBiotin	YEQDPWGVKWWYGGGSKK(K-Biotin)	2,524.86	2,524.21	Original sequence
AcM2pepBiotin	Ac-YEQDPWGVKWWYGGGSKKK(K-Biotin)	2,694.77	2,693.32
W10w	Ac-YEQDPWGVKwWYGGGSKKK(K-Biotin)	2,694.77	2,694.04
W(10,11)w	Ac-YEQDPWGVKwwYGGGSKKK(K-Biotin)	2,694.77	2,693.83
W10P	Ac-YEQDPWGVKPWYGGGSkkk(K-Biotin)	2,605.67	2,605.47
W10Y	Ac-YEQDPWGVKYWYGGGSkkk(K-Biotin)	2,671.73	2,671.02
K9R	Ac-YEQDPWGVRWWYGGGSKKK(K-Biotin)	2,722.78	2,721.22
AcM2pep(RY)Biotin	Ac-YEQDPWGVRYWYGGGSkkk(K-Biotin)	2,699.74	2,699.45	Supplementary info.
W10(P,D,T,R,H) (Crude mixture)	Ac-YEQDPWGVK(P,D,T,R,H)WYGGGSkkk(K-Biotin)	P: 2,605.67 D: 2,623.64 T: 2,609.66 R: 2,664.74 H: 2,645.70	P:2,604.96 D:2,624.78 T:not detected R:2,664.17 H:2,646.17	Supplementary info.
Y12y	Ac-YEQDPWGVRYWyGGGSkkk(K-Biotin)	2,699.74	2,698.65	.
P5Hyp	Ac-YEQD(Hyp)WGVRYWyGGGSkkk(K-Biotin)	2,715.74	2,715.00
R0	Ac-RYEQDPWGVRYWyGGGSkkk(K-Biotin)	2,855.93	2,856.18
Cyclic M2pepBiotin analogs
Cyclic M2pep(RY)Biotin	CGYEQDPWGVRYWYGCkkk(K-Biotin)	2,718.16	2,717.59	GGGS spacer to GC
cRYWY	CGDPWGVRYWYGCkkk(K-Biotin)	2,297.74	2,297.25	GGGS spacer to GC Deletion of Y1E2Q3
cRYW	CGDPWGVRYWGCkkk(K-Biotin)	2,134.56	2,134.00	GGGS spacer to GC Deletion of Y1E2Q3, Y12
Sulfo-Cy5-labeled peptides
M2pep-sulfoCy5	YEQDPWGVKWWYGGGSKKK(k(N3)-sulfoCy5)	3,132.74	3,132.17
Cyclic M2pep(RY)-sulfoCy5	CGYEQDPWGVRYWYGCkkk(k(N3)-sulfoCy5)	3,197.86	3,198.13

All peptides are amidated at the C terminus. Ac denotes N-terminal acetylation. Small letters denote d-amino acids.

Amino acid positions are in reference to the original M2pepBiotin sequence.

Modifications to the original M2pepBiotin are highlighted in red.

Mw observed from MALDI-TOF MS is reported as [M+H]+.