Table 1.
Name | Sequence | Mw (Calculated) | Mw (Observed) | Note |
---|---|---|---|---|
Linear M2pepBiotin analogs | ||||
M2pepBiotin | YEQDPWGVKWWYGGGSKK(K-Biotin) | 2,524.86 | 2,524.21 | Original sequence |
AcM2pepBiotin | Ac-YEQDPWGVKWWYGGGSKKK(K-Biotin) | 2,694.77 | 2,693.32 | |
W10w | Ac-YEQDPWGVKwWYGGGSKKK(K-Biotin) | 2,694.77 | 2,694.04 | |
W(10,11)w | Ac-YEQDPWGVKwwYGGGSKKK(K-Biotin) | 2,694.77 | 2,693.83 | |
W10P | Ac-YEQDPWGVKPWYGGGSkkk(K-Biotin) | 2,605.67 | 2,605.47 | |
W10Y | Ac-YEQDPWGVKYWYGGGSkkk(K-Biotin) | 2,671.73 | 2,671.02 | |
K9R | Ac-YEQDPWGVRWWYGGGSKKK(K-Biotin) | 2,722.78 | 2,721.22 | |
AcM2pep(RY)Biotin | Ac-YEQDPWGVRYWYGGGSkkk(K-Biotin) | 2,699.74 | 2,699.45 | Supplementary info. |
W10(P,D,T,R,H) (Crude mixture) |
Ac-YEQDPWGVK(P,D,T,R,H)WYGGGSkkk(K-Biotin) | P: 2,605.67 D: 2,623.64 T: 2,609.66 R: 2,664.74 H: 2,645.70 |
P:2,604.96 D:2,624.78 T:not detected R:2,664.17 H:2,646.17 |
Supplementary info. |
Y12y | Ac-YEQDPWGVRYWyGGGSkkk(K-Biotin) | 2,699.74 | 2,698.65 | . |
P5Hyp | Ac-YEQD(Hyp)WGVRYWyGGGSkkk(K-Biotin) | 2,715.74 | 2,715.00 | |
R0 | Ac-RYEQDPWGVRYWyGGGSkkk(K-Biotin) | 2,855.93 | 2,856.18 | |
Cyclic M2pepBiotin analogs | ||||
Cyclic M2pep(RY)Biotin | CGYEQDPWGVRYWYGCkkk(K-Biotin) | 2,718.16 | 2,717.59 | GGGS spacer to GC |
cRYWY | CGDPWGVRYWYGCkkk(K-Biotin) | 2,297.74 | 2,297.25 | GGGS spacer to GC Deletion of Y1E2Q3 |
cRYW | CGDPWGVRYWGCkkk(K-Biotin) | 2,134.56 | 2,134.00 | GGGS spacer to GC Deletion of Y1E2Q3, Y12 |
Sulfo-Cy5-labeled peptides | ||||
M2pep-sulfoCy5 | YEQDPWGVKWWYGGGSKKK(k(N3)-sulfoCy5) | 3,132.74 | 3,132.17 | |
Cyclic M2pep(RY)-sulfoCy5 | CGYEQDPWGVRYWYGCkkk(k(N3)-sulfoCy5) | 3,197.86 | 3,198.13 |
All peptides are amidated at the C terminus. Ac denotes N-terminal acetylation. Small letters denote d-amino acids.
Amino acid positions are in reference to the original M2pepBiotin sequence.
Modifications to the original M2pepBiotin are highlighted in red.
Mw observed from MALDI-TOF MS is reported as [M+H]+.