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FusBLU assay for rapid TMPRSS2-ERG detection. Total RNA is isolated from urine and TMPRSS2-ERG mRNA is specifically amplified by RT-RPA isothermally. During strand polymerization, biotinylated dUTP bases are randomly incorporated and subsequently, SA-magnetic beads and SA-HRP are added to RT-RPA products for coupling through biotin-SA interactions. After magnetic isolation of RT-RPA products, TMB is added and a blue color change indicates the presence of TMPRSS2-ERG.
