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Specificity of FusBLU assay. (A) HRP-catalyzed colorimetric results for DuCap (fusion positive) and LnCap (fusion negative) cell lines with corresponding gel electrophoresis images after RT-RPA. Top panel: TMPRSS2-ERG. Bottom panel: RN7SL1. No reverse transcriptase (No RT) and no-template control (NTC) were included. (B) TMPRSS2-ERG levels normalized to RN7SL1. Error bars represent standard deviation of three independent experiments.
