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. 2016 Jun 15;6(9):1415–1424. doi: 10.7150/thno.15250

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Sensitivity of FusBLU assay. (A) HRP-catalyzed colorimetric change generated from 10²- 10⁸ copies of synthetic TMPRSS2-ERG mRNA, and corresponding gel electrophoresis images after RT-RPA. (B) Calibration plot of the average absorbance measurements at different initial RT-RPA input amounts. Inset shows the analogous linear calibration plot. (C) HRP-catalyzed current change generated from 10³ - 10⁷ copies of synthetic TMPRSS2-ERG mRNA. Inset shows the analogous linear calibration plot. Error bars represent standard deviation of three independent experiments.