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Source of TMPRSS2-ERG mRNA in urine. Total RNA isolated from circulating free, sediment, and exosomal fractions of 3 patient urine samples were studied (A) HRP-catalyzed colorimetric changes and corresponding gel electrophoresis images after RT-RPA. Top panel: for TMPRSS2-ERG. Bottom panel: RN7SL1. (B) TMPRSS2-ERG levels normalized to RN7SL1. Error bars represent standard deviation of three independent experiments.
