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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Virology. 2016 Apr 22;494:129–142. doi: 10.1016/j.virol.2016.04.012

Fig. 5.

Fig. 5

Regulation of HTLV replication by NFARs. (A) HTLV infection does not induce NFAR relocalisation. HeLa cells were transfected with 750 ng HTLV proviral clones pACH (HTLV-1) or pH6neo (HTLV-2) (green). HTLV p24 capsid proteins were detected using an antibody against HTLV-2 p24, followed by Alexa Flour 488® staining. Endogenous NFARs were stained as in Fig. 3A. (B–C) YM155 inhibits HTLV replication. MT2 and Mo cells were treated with 100 nM of YM155 (control: 0.2% DMSO). 48 h post-YM155 treatment, cellular supernatants were collected and cells were lysed in RIPA buffer. P19gag levels in the supernatants were quantified by ELISA and cellular lysates were immunoblotted with anti-p24gag, anti-survivin and anti-α-tubulin antibodies. The average of three independent experiments ± standard deviation is shown. Data are represented as percentage change compared to the P19gag levels obtained for YM155-untreated cells which was set arbitrarily to 100%. Asterix (**) indicates statistical significance of p = <0.01 obtained by two-tailed Student’s t-test.